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Title   타목시펜에 의한 인체 유방암세포주의 Apoptosis 에서 bcl - 2 와 p53 단백의 역할 ( The Role of bcl - 2 and p53 in Tamoxifen - Induced Apoptosis of Human Breast Cancer Cell Lines )
Publicationinfo   2000 Jan; 032(03): 531-539.
Key_word   Apoptosis , bcl - 2 protein , Breast neoplasm , p53 protein , Tamoxifen
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Abstract   Purpose: Tamoxifen has been well known as an effective anti-tumor agent against breast cancer. The important role of bcl-2 and p53 proteins in tamoxifen-induced apoptosis of breast cancer cells has been suggested. However, the paradoxical fact that bcl-2 over-expression is associated with better prognosis in clinic has not yet been clearly explained. To investigate this paradox, we analyzed the effect and dynamics of bcl-2 and p53 on the apoptosis after treatment of breast cancer cells with tamoxifen. Materias and Methods: The human breast cancer cell lines MCF-7 and MB MDA-468 were treated with 17- βestradiol (E2) and tamoxifen. Results: Following tamoxifen treatment, MCF-7 cells underwent apoptosis accompanied by reduced bcl-2 expression. E2 pre-treatment led to the inhibition of tamoxifen-mediated apoptosis and bcl-2 down-regulation. When MB MDA-468 cells were treated with E2 or tamoxifen, bcl-2 and p53 protein expression did not change and apoptosis did not develop. Conclusion: We observed that the down-regulation of bcl-2 by tamoxifen treatment can facilitate the apoptosis of breast cancer cells without p53 mutations. This finding was consistent with clinical experiences in which bcl-2 positive tumors were associated with more indolent phenotypes in breast cancer.
저 자   최국진(Kuk Jin Choe),노우철(Woo Chul Noh),노동영(Dong Young Noh),함용호(Yong Ho Ham),김창민(Chang Min Kim),백남선(Nam Sun Paik),문난모(Nan Mo Moon)